NettetThermo Scientific Pierce Hoechst 33342 Fluorescent Stain is a high-quality solution of Hoechst dye for fixed- and live-cell fluorescent staining of DNA and nuclei in cellular … NettetHoechst 33342 is a DNA staining dye that exhibits low cytotoxicity. It fluoresces blue and is used as an indicator of total cell count. Trypan Blue Cell Counting Trypan Blue is one of several stains recommended for use in dye exclusion procedures for viable cell counting.
Cell Viability and Proliferation Assays - Sigma-Aldrich
NettetStaining is a technique used to enhance contrast in samples, generally at the microscopic level. Stains and dyes are frequently used in histology (microscopic study of biological … gender segmentation business examples
Live Imaging of Planaria SpringerLink
Nettet1. sep. 2016 · Dyes that bind to DNA, such as Hoechst 33342 or 4',6-diamidino-2-phenylindole (DAPI), can be used to observe nuclear condensation. These dyes fluoresce at 461 nm when excited by ultraviolet light and can therefore be visualized using conventional fluorescent microscopes equipped with light sources that emit light at … Nettet(for excitation of QDot, wtGFP, Hoechst Blue and DAPI) 9 . Epi White Light Excitation . Turns “ON” or “OFF” the visible overhead white lighting (for focusing in live preview and opaque colony plates) 10 . UV Transilluminator . Controls the intensity of the 300 nm UV transilluminator; (use high intensities for imaging and lower ... NettetFigure 1. Live cell imaging of cellular mitochondria. A) Live HeLa cells co-stained with BioTracker NIR650 Lysosome Dye (SCT139) and BioTracker 488 Green Mitochondria Dye (SCT136).B) Live HeLa cells co-stained with BioTracker ATP-Red live cell dye (SCT045), Hoechst (94403) and a green mitochondria-specific dye showing localization within … gender sector working group