Pcrxl-topo
SpletRoom temperature ligation (5 min) using the pCRXL-TOPO (Invitrogen-Life Technologies Inc., Carlsbad, CA) plasmid was performed and DH5a (Invitrogen-Life Technologies Inc.) were transformed by heat shock. Kanamycin resistant colonies were PCR screened using the EGFssF and NEWPLCR2 primers and the colonies as template using the above … Splet20. maj 2024 · The PCR product was cloned into pCRXL-TOPO (Invitrogen, Thermo Fischer Scientific, Illkirch, France), and the recombinant plasmid (pCRXL-TOPO:msrA) was …
Pcrxl-topo
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SpletThe TOPO™ XL-2 Complete PCR Cloning Kit provides all the necessary elements for efficient cloning of extra-long PCR products (up to 13 kb). The kit uses the linearized and … SpletpCR2.1-TOPO. Parental bacterial expression vector for TOPO® TA cloning of PCR products. Sequence Author: Thermo Fisher (Invitrogen) Open in SnapGene. Try SnapGene for Free. …
Splet丁香通为您找到165条点突变pcr跑胶没有条带信息，包括点突变pcr跑胶没有条带报价行情，优质供应商，图片，品牌等最新信息，丁香通为买家提供用户服务，诚信保障等服务，批发采购点突变pcr跑胶没有条带,上丁香通。 Spletcloned into pCRXL-TOPO or pCR2.1-TOPO cloning vector individually (Invitrogen, USA). The colonies were selected on LB medium supplemented with kanamycin (50 mg/ml) after transformation into E.coli DH5a cells. The recombinant clones containing the in-dividual pectolytic genes from XW19 or XW121 were sequenced using an automatic DNA …
SpletpCR-XL-TOPO (linearized) Linearized vector with 3'-T overhangs and bound topoisomerase, for TOPO® TA cloning of long PCR products. Positive clones disrupt a lethal lacZα-ccdB … Splet质粒载体网zlzt.com专业提供pCR-XL-TOPO-SLC24A1(human) ,产品编号为zl-008487,为科研单位及院所提供的质粒载体展示及购买平台,保藏了大量质粒载体,致力于完美质粒众筹,质粒合成,质粒构建,质粒提取,质粒保藏!
SpletThe TOPO™ XL-2 Complete PCR Cloning Kit provides a highly efficient one-step method ("TOPO™ Cloning") for the cloning of extra long PCR products generated by the …
SpletDownload pCR-XL-2-TOPO.dna file Download Plasmid Open in SnapGene SnapGene SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures Fast accurate construct design for all major molecular cloning techniques Validate sequenced constructs using powerful alignment tools bones tv show therapistSplet01. okt. 2002 · The product of the inverse PCR was gel extracted, cloned with the TOPO TA Cloning kit with vector pCRXL-TOPO (Invitrogen), and fully sequenced. Primers NppkI-F and NppkI-R were subsequently designed to target the full-length Type I ppk (Table 2 ). gobby beachSpletTOPO™ TA Cloning™ Kits are designed for cloning PCR products directly from a PCR reaction in just 5 minutes (1). They use a pCR™-TOPO™ Vector with covalently bound … gobby lobby.comSplet20. feb. 2024 · The kit uses the linearized and topoisomerase 1-activated pCR™-XL-2-TOPO™ vector, which is compatible with the cloning of blunt-end PCR fragments. Amplification of long PCR fragments is enabled by Platinum™SuperFi™ Green PCR Master Mix, which is included in the kit. Topoisomerase I activation of the vector allows PCR … bones tv show season 12 castSpletVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene. This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details. bones \u0026 chewsSpletpCR-XL-TOPO Parental vector for TOPO® TA cloning of long PCR products. Vector Map (Click image to enlarge) Search our products with this vector backbone We offer a wide … gobby lipsSplet05. dec. 2015 · Chimeric Rhinoviruses Obtained via Genetic Engineering or Artificially Induced Recombination Are Viable Only if the Polyprotein Coding Sequence Derives from the Same ... bones tv show when does brennan love booth