Phospho antibody
WebAnti-Phospho Antibody HRP-Conjugated Secondary Antibody Assay Diluent TMB One-Step Substrate Stop Solution Lysis Buffer Positive Control Sample Other Materials Required Distilled or deionized water 100 ml and 1 liter graduated cylinders Tubes to prepare sample dilutions Protease and Phosphatase inhibitors
Phospho antibody
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WebThe cells are stimulated, fixed, and blocked in the same well. Phospho-specific antibodies are used to assess phosphorylation status using fluorometric (Figure 4) or colorimetric detection systems. Furthermore, the phospho-protein and total protein are simultaneously detected in the same microplate well. WebRecombinant Anti-JAK1 (phospho Y1034 + Y1035) antibody [EPR1899 (2)] - BSA and Azide free (ab203784) Datasheet Certificate of Compliance Reviews ( 1) Submit a question References (2) $8,985.00 Product size 100 µg $905 1 mg $8,985.00 Add to basket Order now and get it on Friday April 14, 2024 Custom quote Bulk request Shipping info
WebTo use the phospho-specific antibodies as an assay for Raf-1 activation, the Raf-1 protein is first immunoprecipitated from cell lysates with an antibody that efficiently immunoprecipitates Raf-1. The immunoprecipitates are then washed, separated by SDS–PAGE, and transferred onto a 0.2-μm pore size nitrocellulose membrane as … WebPhospho-mTOR (Ser2448) Antibody detects endogenous levels of mTOR only when phosphorylated at Ser2448. Species Reactivity: Human, Mouse, Rat, Monkey Species …
WebThis recombinant antibody clone is derived from clone A18028A. This clone is predicted to recognize the region corresponding to EGFR Phospho (Tyr845) in both rat (Tyr846) and mouse (Tyr847) orthologs due to complete sequence conservation with the immunizing peptide. Antigen Details Structure WebPhospho-S6 (Ser235, Ser236) Antibody (MA5-15140) in ICC/IF. Immunofluorescence analysis of Phospho-S6 (Ser235,Ser236) was performed using 70% confluent log phase U …
WebAnti-Phospho - (Ser/Thr) antibody Description Rabbit polyclonal to Phospho - (Ser/Thr) Host species Rabbit Tested applications Suitable for: ELISA, Dot blot more details Species …
WebThis protocol describes how to detect phosphorylated proteins by western blotting using Phospho-Specific Validated PrecisionAb Antibodies. We also provide a list of reagents … how to start car with faulty starterWebPhospho-(Ser/Thr) Phe Antibody detects phospho-serine or threonine in the context of tyrosine, tryptophan or phenylalanine at the -1 position or phenylalanine at the +1 position. The antibody does not cross-react with … react contenteditable placeholderWebAll lanes : Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) at 1/1000 dilution Lane 1 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) Whole … how to start car with screwdriverWebFeb 26, 2024 · p-Ser Antibody (16B4) is an IgM κ mouse monoclonal p-Ser antibody (also designated phosphorylated serine antibody, phospho serine antibody, or p-serine antibody) that detects the p-Ser protein of mouse, rat, human and canine origin by WB and IP. p-Ser Antibody (16B4) is available as the non-conjugated anti-p-Ser antibody. react context dispatch typescriptWebKey features and details. Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply. Rabbit monoclonal [EPR1899 (2)] to JAK1 … react contenteditable editorWebEach phospho-specific antibody has been tested in three different flow cytometry buffer systems: IC Fixation and Permeabilization , Foxp3/Transcription Factor Buffer, and IC … react context for authenticationWebFor those antibodies which are suspected to be more intensely/prominently binding, one can or advised to dilute it in 5% NFM (non-fatty milk) But there are antibodies which are very weakly... react context displayname