WebApr 4, 2011 · The F14Bpa gene with C-terminal 6×His tag was cloned into a pET15b vector using restriction sites Nco I and BamH I. A pSup-BpaRS-6TRN 29 vector harboring the …

Dynamic Interaction of the Sec Translocon with the Chaperone PpiD

Web(DE3) cells harboring vectors pSUP-BpaRS-6TRN, pCDF-ftsBLmat,andoneofthep29SENX-SH 8 ftsQvariantsweregrown in 25 ml of growth medium, and when an A 600 of 0.3 was reached, Bpa was added to 0.5 mM. After 20 min of continued growth, isopropyl 1-thio- -D-galactopyranoside was added to 0.5 mM, and the cells were grown for a further 1.5 h. The … WebpSup-BpaRS-6TRN(D286R) were further transformed with pSSl encoding the wild-type or amber mutants of LolA and pSS24 encoding mLolB, and then grown in a medium supple-mented with pBPA and isopropyl-ß-D-thiogalactopyranoside (IPTG). The cultures were irradiated with UV light for 5 min, and then analyzed by SDS/PAGE and immunoblotting. … mlb station tv schedule

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WebApr 4, 2011 · The F14Bpa gene with C-terminal 6×His tag was cloned into a pET15b vector using restriction sites Nco I and BamH I. A pSup-BpaRS-6TRN 29 vector harboring the stop codon suppressor tRNA was co-transformed into BL21 (DE3) cells for expression of F14Bpa. Bpa was added into 2YT media to a final concentration of 2 mM. WebJan 21, 2011 · The method for overexpression and purification of LptD/E was as previously reported , except that each BL21(DE3) overexpression strain harbored pSup-BpaRS-6TRN … WebProtein Expression and Purification—The plasmid pSup-BpaRS-6TRN, expressing the orthogonal tRNA CUA /pBpa-tRNA synthetase pair (15), was co-transformed into E.coli strain OverExpressTM C41 (DE3) (Lucigen) together with mutant pSup(1–61)(4M2A)-GFP-Strep(II)-His 6 plasmids, whichexpressedpBpa-containingproteins.Thetransformants mlb station schedule